خدمات پروتئومیکس > خدمات آزمون پروتیئن
Protein Analytical Service
Available method | Analysis target | Analytical services | |
---|---|---|---|
Western Blot | Protein identification | Primary structure |
Identification & characterization |
MALDI-TOF MS/LC-MS/SDS-PAGE/SEC-HPLC/CE-SDS | Molecular weight | ||
RP-HPLC-UV | Peptide mapping | ||
LC-MS/MS | Sequence coverage | ||
LC-MS/MS | N-terminal/C-terminal sequence identification | ||
LC-MS/MS | Disulfide bond identification | Post translational modifications - PTM | |
LC-MS/MS | N-glycosylation identification | ||
HILIC-HPLC/MS | N-glycan analysis | ||
LC-MS/MS/HIC-HPLC | PTM analysis | ||
CD | Higher order | Higher order structures | |
DSC | Tm of CH2,CH3 | ||
IEF/cIEF | pI | Others | |
cIEF/IEC-HPLC | Charge variants | ||
RP-HPLC-UV/FLD | Amino acid analysis | ||
SDS-PAGE/SEC-HPLC/CE-SDS | Purity | Purity & quantitation | |
Bradford/Lowry/BCA/UV/ELISA/AC-HPLC(protein A) | Quantitation | ||
qPCR/SYBR green method | Host cell DNA-HCD | Impurity | |
ELISA | Host cell Protein-HCP | ||
ELISA | Residual Protein A | ||
Tal reagent | Bacterial Endotoxins | Safety & Stability |
|
Membrane filtration | Microbial limit | ||
Membrane filtration | Sterile examination | ||
Animal test (mouse) | Abnormal toxicity | ||
PCR | Mycoplasma | ||
Light obscuration test | Insoluble | ||
Lamp test | Visible particles | ||
DSC | Denaturation Temperature |
لطفاً به منظور درک بیشتر به نمونه های واقعی زیر دقت فرمایید:
Figure 1. Original mass spectrum
Figure2. Deconvoluted mass spectrum
Figure 3. Deconvoluted mass spectrum after deglycosylation
Case 2: Glycan analysis by HPLC-MS
Sample: Antibody
Sample type: solution in PBS
LC method: HILIC-HPLC column, 65 min Gradient
Scan Type: TOF MS
Reagent Kits from Waters
Figure 4. N-glycan types of antibodies - Trends in biotechnology, Volume 34, issue 10, p835-846
Figure 5. Total ion chromatogram of N-glycan by LC-MS method
Figure 6. 20+ N-glycan types identified in the table
جهت سفارش، لطفاً فرم مربوط را از اینجا دریافت نمایید و پس از تکمیل آنرا برای ما ارسال فرمایید.