خدمات پروتئومیکس > خدمات آزمون پروتیئن

 

Protein Analytical Service  

Available method Analysis target Analytical services
Western Blot Protein identification Primary structure

Identification & characterization

MALDI-TOF MS/LC-MS/SDS-PAGE/SEC-HPLC/CE-SDS Molecular weight
RP-HPLC-UV Peptide mapping
LC-MS/MS Sequence coverage
LC-MS/MS N-terminal/C-terminal sequence identification
LC-MS/MS Disulfide bond identification Post translational modifications - PTM 
LC-MS/MS N-glycosylation identification
HILIC-HPLC/MS N-glycan analysis
LC-MS/MS/HIC-HPLC PTM analysis
CD Higher order Higher order structures
DSC Tm of CH2,CH3
IEF/cIEF pI Others
cIEF/IEC-HPLC Charge variants
RP-HPLC-UV/FLD Amino acid analysis
SDS-PAGE/SEC-HPLC/CE-SDS Purity Purity & quantitation
Bradford/Lowry/BCA/UV/ELISA/AC-HPLC(protein A) Quantitation
qPCR/SYBR green method Host cell DNA-HCD Impurity
ELISA Host cell Protein-HCP
ELISA Residual Protein A
Tal reagent Bacterial Endotoxins Safety & Stability
 
Membrane filtration Microbial limit
Membrane filtration Sterile examination
Animal test (mouse) Abnormal toxicity 
PCR Mycoplasma
Light obscuration test Insoluble 
Lamp test Visible particles
DSC Denaturation Temperature

 

 


 

 لطفاً به منظور درک بیشتر به نمونه های واقعی زیر دقت فرمایید:


Case Study 1: Sequence coverage by LC-MS
Case sample: Antibody
Digestion: Trypsin, overnight
LC method: C18 column, 40 min Gradient
MS/MS mode: IDA
Data analysis: Protein pilot 5.0

 

Figure 1. Original mass spectrum 



Figure2. Deconvoluted mass spectrum


 

Figure 3. Deconvoluted mass spectrum after deglycosylation



 


Case 2: Glycan analysis by HPLC-MS

Sample: Antibody

Sample type: solution in PBS

LC method: HILIC-HPLC column, 65 min Gradient

Scan Type: TOF MS

Reagent Kits from Waters



Figure 4. N-glycan types of antibodies - Trends in biotechnology, Volume 34, issue 10, p835-846



Figure 5. Total ion chromatogram of N-glycan by LC-MS method



Figure 6. 20+ N-glycan types identified in the table

 

 جهت سفارش، لطفاً فرم مربوط را از اینجا دریافت نمایید و پس از تکمیل آنرا برای ما ارسال فرمایید.